Figure 3.

Opposite effects of AIF-1 and IRT-1 on VSMC proliferation and migration. (A) Western blots confirming overexpression of AIF-1 (upper panel) or IRT-1 (lower panel) in infected rat VSMCs. GAPDH was used to verify equal loading. (B) Effect of AdAIF-1 and AdIRT-1 overexpression on VSMC proliferation. Means are from three independent experiments performed in triplicate. (C) Migration of VSMCs infected with AdAIF-1, AdIRT-1, or AdGFP in response to PDGF-AB (40 ng) for 3 h. The mean number of migrated cells per high-power field (HPF) from three independently infected groups performed in triplicate are shown. *P< 0.05 and **P< 0.01 vs. AdGFP.