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. 2011 Nov 23;93(3):414–423. doi: 10.1093/cvr/cvr309

Figure 5.

Figure 5

NFATc3 inhibits IRT-1 expression. (A) Human VSMCs were incubated for 2 days in 15% FBS, with or without A-285222 (1.0 μM). Proliferation was measured by MTS assay and normalized to control (n= 4). (B) Representative western blot and summarized data from experiments showing IRT-1 protein levels in human VSMCs treated as in (A). Data were normalized to GAPDH and expressed as percentage of control (n= 4). (C and D) Summarized data from western blot experiments showing NFATc3 (C) and IRT-1 (D) expression in human VSMCs 5 days after transfection with siNFATc3 (100 nM). Representative blots from the same gel show simultaneous down-regulation of NFATc3 and up-regulation of IRT-1 expression. Data were normalized to β-actin and expressed as percentage of control (siCONTROL, n= 5). *P < 0.05 and ***P < 0.001 vs. controls.