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. 2011 Mar 1;2(3):471–476. doi: 10.3892/ol.2011.261

Figure 2.

Figure 2

UVB-induced apoptosis of MCF-7 cells detected by flow cytometry was potentiated by 5-fluorouracil (5-FU). The population of Annexin V+ PI+/− apoptotic cells was evaluated by flow cytometry using Annexin V-FITC/PI staining in MCF-7 cells, treated in the absence or presence of 5-FU (10 or 20 μM) without or with UVB-irradiation (750 mJ/cm2). Treatment with 5-FU alone caused a slight increase in the percentage of apoptotic cells (12.2 vs. 15.2 and 17.2%; untreated vs. treated with 5-FU at 10 and 20 μM). UVB-irradiation alone also caused a partial increase in apoptotic cells (12.2 vs. 39.8%; untreated vs. UVB alone), while the combination treatment of 5-FU and UVB resulted in a significant increase in apoptotic cells (39.8 vs. 62.2 and 74.1%; UVB alone vs. UVB + 5-FU at 10 and 20 μM). The experiment was performed three times, and the ratio of apoptotic cells was expressed as mean ± SD. *P<0.05.