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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Exp Mol Pathol. 2012 Apr 16;93(1):74–81. doi: 10.1016/j.yexmp.2012.04.004

Figure 3. Effect of TNF-α + Calcitriol on mRNA and protein expression of VDR in HBSMCs: A-B.

Figure 3

Cultured HBSMCs were serum starved for 24 hr followed by treatment with TNF-α (10ng/ml) ± calcitriol (100 nM) for 24 hr. The mRNA and protein were isolated from whole cell lysate and subjected to qPCR and immunoblotting, respectively. Data is shown as mean ± SEM from three individual samples in each experiment; *p <0.05, **p<0.01, ***p<0.001. C: HBSMCs cultured in chamber slides were quiesced before addition of TNF-α (10ng/ml) ± calcitriol (100 nM) for 24 hours. HBSMCs were fixed, permeabilized and stained with rabbit anti-VDR and goat anti-rabbit cy3 as secondary antibody. Red color indicates VDR expression and nuclei are stained blue with DAPI.