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. Author manuscript; available in PMC: 2012 Aug 2.
Published in final edited form as: Nutr Cancer. 2011 Dec 9;64(1):163–172. doi: 10.1080/01635581.2012.630168

Table 2.

Selected reaction monitoring (SRM) transitions for chiral LC-MS/MS analysis of R- and S-HETE enantiomers

Compound [M–H]–
(m/z)		 SRM Transition Collision Energy
(eV)		 Tube Lens
(V)
5-R/S-HETEs† 319 319.5 → 115.27 27 −42
5- S-HETE-d8 327 327.50 → 116.30 28 −102
12- R/S-HETEs† 319 319.24 → 179.09 26 −55
12-S-HETE-d8 327 327.24 → 184.09 22 −55
15- R/S-HETEs† 319 319.31 → 219.30 20 −52
15- S-HETE-d8 327 327.20 → 226.05 20 −52
†

In chiral LC, the R- and S-enantiomers of each HETE are resolved and elute as 2 distinct peaks. Both peaks for each HETE were quantified relative to the respective S-HETE-d8 internal standard.