Figure 5.

Assessment of squamous-cell differentiation in OTC with genetically engineered human esophageal epithelial cell lines with or without DNMAML1. Cell lines with indicated genotypes expressing either DNMAML1 or GFP (control) were grown in OTC to reconstitute stratified squamous epithelia. Serial sections from the paraffin embedded end products from Figure 2A were subjected to IF for CK14 (red) and IVL (green) with DAPI (blue). Parental, EPC2-hTERT with empty vectors only; EGFR, EGFR overexpression; p53, p53^R175H; and D1, cyclin D1 overexpression. Epi, epithelium; Str, stroma; Scale bar, 50 μm.