Figure 1.

RWJ67657 inhibits basal p38 signaling pathway in tamoxifen resistant breast Cancer. (A) MDA-MB-361 cells were treated with increasing concentrations of RWJ67657 (0.1 μM, 1.0 μM, or 10 μM). Lysates were assayed by western blot using antibodies directed against phosphorylated p38 (pp38), total p38 (t-p38), phosphorylated hsp27 (phsp27), or total hsp27 (t-hsp27). The blots shown are representative of three independent experiments. (B) Densitometry analysis of protein levels. Quantified phosphorylated protein bands were divided by total protein bands within each lane and total protein load was normalized to Rho-GDI levels. The vehicle lane of each protein was set to 100% for comparative purposes. Data points and error bars represent the mean ± S.E.M. of three independent experiments in duplicate (*** p < 0.001, ** p < 0.01, *p < 0.05).