Figure 3.

Pharmacological inhibition of p38 blocks ER transcription activity in vitro. MDA-MB-361 cells were grown in charcoal stripped media for 48 hours. The cells were then transfected with an ERE-luciferase reporter construct, followed by the addition of vehicle, 10 pM estradiol (E2), or E2 plus increasing concentrations of RWJ67657 (0, 1, 2, 4, and 10 μM). Assays were performed in triplicate. Results were normalized to E2 control. Data points and error bars represent the mean ± S.E.M. of three independent experiments in duplicate (*** p < 0.001, ** p < 0.01, *p < 0.05).