. 2012 Aug 2;3:148. doi: 10.3389/fphar.2012.00148

Table 3.

Kinetic constants of AKR1B toward 4-hydroxynonenal, isocaproaldehyde, and prostaglandin H2.

Substrates	4-Hydroxynonenal		Isocaproaldehyde		Prostaglandin H2^e
	K_m (μM)	K_cat (s⁻¹)	K_m (μM)	K_cat (s⁻¹)	K_m (μM)	V_max (nmol/min/mg)
HUMAN
AKR1B1	716^a	0.84^a	1^b	0.66^b	1.9	44
AKR1B10	31^a	2.01^a	330^c	0.72^c	No activity
AKR1B15	n.d.	n.d.	n.d.	n.d.	n.d.
MOUSE
Akr1b3	665^a	0.82^a	62^d	1.3^d	9.3	26
Akr1b7	256^a	0.1^a	320^d	0.38^d	3.8	53.4
Akr1b8	230^a	3.18^a	71^d	0.03^d	No activity
Akr1b16	n.d.	n.d.	n.d.	n.d.	n.d.
RAT
Akr1b4	33^f	0.23^f	n.d.	n.d.	n.d.
r-Akr1b10	1.6^g	0.05^g	11^g	0.03^g	n.d.
Akr1b13	30^f	0.18^f	n.d.	n.d.	n.d.
Akr1b14	7.6^h	0.02^h	16^h	0.03^h	n.d.

n.d., not determined.

References (for comparison data were converted in μM for the K_m and in s⁻¹ for the K_cat): ^aJoshi et al. (2010), ^bMatsuura et al. (1996), ^cMartin and Maser (2009), ^dMartinez et al. (2001), ^eKabututu et al. (2009), ^fEndo et al. (2009a), ^gEndo et al. (2010b), ^hEndo et al. (2010a).