Figure 4.

Heat map representing the changes in relative metabolite contents of treated and control Arabidopsis leaf disks. Leaf disks were cut directly from 4-week-old plants, washed three times with 10 mM MES-KOH (pH 6.5), and then incubated during up to 4 h in 10 mM MES-KOH buffer (pH 6.5) containing 2.0 mM glucose and 100 μM of SP or CESP. Metabolites were determined as described in the Section “Materials and Methods.” Data are normalized with respect to the mean response calculated for the control at 2 h. The full data sets from these metabolic profiling studies are available as Table A1 in Appendix including statistical treatment. Green and red represent a decrease and an increase of metabolite content, respectively, in the SP-treated samples with respect to the control samples. The color scale used is reproduced in the Figure.