Table 1.
Assays of the key enzymes related to the TCA cycle in the absence (Control) and presence of 100 μM of SP or CESP.
| Control | SP | CESP | |
|---|---|---|---|
| nmol min−1 g−1 FW | |||
| Aconitase | 41.5 ± 4.6 | 39.9 ± 5.4 | 40.4 ± 6.3 |
| Citrate synthase | 165.8 ± 12.6 | 160.1 ± 14.5 | 173.4 ± 10.1 |
| Fd-GOGAT | 1157.5 ± 94.4 | 1203.9 ± 111.3 | 1099.9 ± 102.7 |
| Glutamate dehydrogenase | 612.8 ± 72.5 | 597.8 ± 86.7 | 614.6 ± 84.7 |
| Glutamine synthetase | 981.9 ± 40.3 | 918.5 ± 59.3 | 938.6 ± 62.6 |
| NAD-isocitrate dehydrogenase | 262.9 ± 25.6 | 246.6 ± 26.2 | 253.2 ± 37.9 |
| Pyruvate kinase | 847.6 ± 64.4 | 873.4 ± 81.4 | 851.6 ± 58.3 |
| Nitrate reductase | 537.5 ± 30.3 | 551.7 ± 41.1 | 541.1 ± 29.9 |
| Succinyl-CoA ligase | 114.5 ± 12.9 | 124.1 ± 12.8 | 115.5 ± 13.7 |
| Pyruvate dehydrogenase | 27.4 ± 2.4 | 24.6 ± 4.4 | 25.7 ± 3.8 |
| μmol min−1 g−1 FW | |||
| Alcohol dehydrogenase | 90.8 ± 3.7 | 89.9 ± 3.3 | 88.1 ± 2.5 |
| Alanine amino transferase | 116.9 ± 5.7 | 103.8 ± 6.7 | 104.7 ± 5.4 |
| Fumarase | 5.6 ± 1.1 | 5.4 ± 0.8 | 4.9 ± 0.9 |
| NAD-malate dehydrogenase | 70.3 ± 8.1 | 72.6 ± 9.8 | 68.2 ± 9.2 |
The in vitro activity of the enzymes were determined in Arabidopsis thaliana leaves extract of fully expanded leaves of 4-week-old plants were harvested at the middle of the light period. Values are presented as the mean ± SE of four replicates.