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. 2012 Jun 4;3:114. doi: 10.3389/fpls.2012.00114

Table 2.

Redistribution of heavy label following glucose feeding of control and treated Arabidopsis leaves.

Control SP CESP
μmol C1 equivalent g−1 FW h−1
Alanine 4.837 ± 1.248 3.989 ± 0.578 4.417 ± 0.707
Asparagine 0.129 ± 0.042 0.117 ± 0.004 0.124 ± 0.014
Aspartate 0.085 ± 0.020 0.060 ± 0.014 0.075 ± 0.015
Fructose 0.479 ± 0.032 0.388 ± 0.107 0.613 ± 0.074
Fumarate 0.231 ± 0.068 0.114 ± 0.013 0.143 ± 0.066
GABA 0.077 ± 0.006 0.115 ± 0.006 0.971 ± 0.007
Glutamate 1.199 ± 0.172 1.712 ± 0.163 1.591 ± 0.134
Glutamine 0.931 ± 0.134 0.679 ± 0.097 0.663 ± 0.180
Histidine 0.992 ± 0.139 0.859 ± 0.045 0.887 ± 0.118
Isoleucine 0.018 ± 0.005 0.015 ± 0.003 0.012 ± 0.003
Lysine 1.477 ± 0.221 1.036 ± 0.128 1.167 ± 0.136
Malate 2.174 ± 0.166 1.522 ± 0.158 1.408 ± 0.206
Methionine 0.044 ± 0.001 0.046 ± 0.001 0.049 ± 0.001
Ornithine 0.364 ± 0.108 0.073 ± 0.015 0.063 ± 0.020
2-Oxoglutarate 0.320 ± 0.168 0.391 ± 0.035 0.383 ± 0.126
Proline 0.029 ± 0.012 0.001 ± 0.001 0.002 ± 0.001
Serine 0.115 ± 0.022 0.087 ± 0.014 0.088 ± 0.018
Sucrose 1.323 ± 0.134 1.184 ± 0.326 1.197 ± 0.255
Valine 0.449 ± 0.109 0.325 ± 0.054 0.341 ± 0.061

Fully expanded leaves of 4-week-old plants were harvested at the middle of the light period, washed three times with 10 mM MES-KOH (pH 6.5), and then incubated during 4 h in similar light growth conditions in 10 mM MES-KOH buffer (pH 6.5) containing 20.0 [U-13C] mM glucose and 100 μM of SP or CESP. Values represent absolute redistribution of the label and are given as means ± SE of determinations on six independent samples. Those set in bold type were determined by the Student’s t-test to be significantly different (P < 0.05) from the control.