Table 2.
Control | SP | CESP | |
---|---|---|---|
μmol C1 equivalent g−1 FW h−1 | |||
Alanine | 4.837 ± 1.248 | 3.989 ± 0.578 | 4.417 ± 0.707 |
Asparagine | 0.129 ± 0.042 | 0.117 ± 0.004 | 0.124 ± 0.014 |
Aspartate | 0.085 ± 0.020 | 0.060 ± 0.014 | 0.075 ± 0.015 |
Fructose | 0.479 ± 0.032 | 0.388 ± 0.107 | 0.613 ± 0.074 |
Fumarate | 0.231 ± 0.068 | 0.114 ± 0.013 | 0.143 ± 0.066 |
GABA | 0.077 ± 0.006 | 0.115 ± 0.006 | 0.971 ± 0.007 |
Glutamate | 1.199 ± 0.172 | 1.712 ± 0.163 | 1.591 ± 0.134 |
Glutamine | 0.931 ± 0.134 | 0.679 ± 0.097 | 0.663 ± 0.180 |
Histidine | 0.992 ± 0.139 | 0.859 ± 0.045 | 0.887 ± 0.118 |
Isoleucine | 0.018 ± 0.005 | 0.015 ± 0.003 | 0.012 ± 0.003 |
Lysine | 1.477 ± 0.221 | 1.036 ± 0.128 | 1.167 ± 0.136 |
Malate | 2.174 ± 0.166 | 1.522 ± 0.158 | 1.408 ± 0.206 |
Methionine | 0.044 ± 0.001 | 0.046 ± 0.001 | 0.049 ± 0.001 |
Ornithine | 0.364 ± 0.108 | 0.073 ± 0.015 | 0.063 ± 0.020 |
2-Oxoglutarate | 0.320 ± 0.168 | 0.391 ± 0.035 | 0.383 ± 0.126 |
Proline | 0.029 ± 0.012 | 0.001 ± 0.001 | 0.002 ± 0.001 |
Serine | 0.115 ± 0.022 | 0.087 ± 0.014 | 0.088 ± 0.018 |
Sucrose | 1.323 ± 0.134 | 1.184 ± 0.326 | 1.197 ± 0.255 |
Valine | 0.449 ± 0.109 | 0.325 ± 0.054 | 0.341 ± 0.061 |
Fully expanded leaves of 4-week-old plants were harvested at the middle of the light period, washed three times with 10 mM MES-KOH (pH 6.5), and then incubated during 4 h in similar light growth conditions in 10 mM MES-KOH buffer (pH 6.5) containing 20.0 [U-13C] mM glucose and 100 μM of SP or CESP. Values represent absolute redistribution of the label and are given as means ± SE of determinations on six independent samples. Those set in bold type were determined by the Student’s t-test to be significantly different (P < 0.05) from the control.