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. 2012 Jun 4;3:114. doi: 10.3389/fpls.2012.00114

Table A2.

Assays of the key enzymes related to fermentation process following inhibition of 2-OGDHC by phosphonate analogous.

Control SP CESP

μmol min−1 g−1 FW
Alcohol dehydrogenase 38.2 ± 4.1 35.9 ± 2.9 41.5 ± 4.1
Alanine amino transferase 98.7 ± 10.7 82.9 ± 17.4 97.5 ± 9.9

Fully expanded leaves of 4-week-old were harvested at the middle of the light period, washed three times with 10 mM MES-KOH (pH 6.5), and then incubated during 4 h in similar light growth conditions in 10 mM MES-KOH buffer (pH 6.5) containing 100 μM of SP or CESP. Values are presented as the mean ±SE of six biological replicates.