Skip to main content
. 2012 Jul 2;3:143. doi: 10.3389/fpls.2012.00143

Figure 10.

Figure 10

Endogenous SYP7 proteins colocalize with ERES and Golgi stacks. (A–C) Immunofluorescent localization of the syntaxin SYP31 to Golgi stacks in transgenic tobacco BY-2 cells expressing the Golgi marker GONST1-YFP. (D–J) Double immunofluorescence on tobacco BY-2 cells using antibodies against the SYP7 family and the COPII protein SEC13 (D–G) or the syntaxin SYP31 (H–J). (K) Western blots showing the specificity of the SYP7 antiserum in total membrane preparations (lanes 1) from Arabidopsis and tobacco leaves (lane 2 is the 100,000-g supernatant as a negative control). (L) Immunogold labeling with SYP7 antibodies of a thin section cut from a high pressure frozen Arabidopsis root tip. Arrowheads point to gold particles. c, cis-Golgi; t, trans-Golgi; NE, nuclear envelope. Magnification bars = 5 μm (A–G); 1 μm (H–J); 300 nm (L).