Figure 3.

The ER marker RFP-p24δ5 forms nodules in the presence of SYP72. Tobacco mesophyll protoplasts were co-electroporated with RFP-p24δ5 (10 μg) and 15 μg of one ER-SNARE [SYP72-GFP (A–C), SYP71-GFP (D–F), CFP-SYP73 (G–I), or YFP-USE1 (J–L)]. In addition to a general staining of the ER network the red RFP-p24δ5 signal is also present as punctae which colocalize exactly with the punctate green SYP72-GFP signals. This phenomenon was partially recognizable in the case of SYP71 and USE1 but was not at all visible with SYP73. All images are of single optical sections. Magnification bars = 5 μm.