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. 2012 Jul 2;3:143. doi: 10.3389/fpls.2012.00143

Figure 4.

Figure 4

Overexpression of SYP72 enhances retrograde Golgi-ER traffic and leads to the formation of p24δ5 nodules in the ER. (A–C) Coexpression of RFP-p24δ5 and SYP72-YFP in agroinfiltrated tobacco leaf epidermal cells leads to production of nodules in the RFP-p24δ5 stained ER tubular network. These nodules label positively with SYP72-YFP (indicated by arrowheads). (D–G) SYP72-YFP and RFP-p24δ5 fluorescence are first detected after 36 h in the nuclear envelope (arrow) and persist for at least 120 h post-infiltration. For images (A–G), the YFP signal is presented in green for easier viewing. (H–J) Coexpression of SYP72-GFP with a mutant form of RFP-p24δ5 (which cannot be exported out of the ER – see Materials and Methods) in tobacco leaf epidermal cells. As with leaf cells expressing only RFP-p24δ5, nodules in the ER tubular network are not present [compare (H) with (I)], and the SYP72-GFP punctate signals are distributed randomly on the surface of the ER (J). Magnification bars = 2 μm (A–C,H); 5 μm (D–G,I,J).