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CFP-HDEL forms nodules only when coexpressed with SYP72-YFP. (A–C) CFP-HDEL expressed alone labels the ER uniformly (A) but forms nodules when coexpressed with SYP72-YFP (B,C) in tobacco leaf epidermal cells. Arrows point to colocalization of nodules with SYP72-YFP punctae. (D–G) Frames of a movie (CFP-HDEL in cyan; SYP72-YFP in yellow) depicting a growing tubule (arrowhead; Movie S1 in Supplementary Material). Magnification bars = 5 μm (A–G).
