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. 2012 Jul 2;3:143. doi: 10.3389/fpls.2012.00143

Figure 6.

Figure 6

Punctate SYP72-YFP signals seldom colocalize with ERES and Golgi markers in normal cells. Tobacco leaves were triple agroinfiltrated with 6 kDa VP-CFP (A), Man1-RFP (B), SYP72-YFP (C). Although a high degree of colocalization between the blue COPII (6 kDa VP-CFP) and red Golgi (Man1-RFP) markers was observed, there was little overlap between the signals for SYP72-YFP (yellow) and the COPII and Golgi markers (D–E). A case of colocalization between SYP72-YFP and Man1-RFP punctae is indicated in the rectangles in (A–C), and in inset 2 in (D) When tobacco leaves were triple agroinfiltrated with 6 kDa VP-CFP, SYP72-YFP, and RFP-p24δ5 a clear distinction can be made between the punctate SYP72-YFP signals which lie directly on the tubular ER network (see insets in (G,H) for nodules) and the Golgi-associated ERES marker [6 kDa VP-CFP; (F–H)]. Magnification bars = 10 μm (A–D,F–H).