Figure 7.

Mobilities and transient colocalization of Man1-RFP and SYP72-YFP as observed in videos of untreated tobacco leaf epidermal cells. (A–H) Eight frames taken at 0.5–1 s intervals from a movie sequence of the SYP72-YFP (shown in green) and Man1-RFP (in red) images (movie available as Movie S2 in Supplementary Material). A Man1-RFP puncta (arrowhead) is seen moving toward and over a SYP72-YFP puncta (star). (I–P) SYP72-YFP (in green) and Man1-RFP (in red) are shown in these eight frames (5 s interval; movie available as Movie S3 in Supplementary Material). Several examples of temporary colocalization between SYP72-YFP and Man1-RFP signals are to be seen (arrowhead). Magnification bars = 5 μm (A–P).