Figure 3.

Hyperexcitability of pyramidal cells following constitutive NMDAR hypofunction is normalized by baclofen. (a) NR1^neo−/− cells demonstrated significant hyperexcitability, measured by the slope of the frequency–current relationship, which was reduced by baclofen (10 μM). Excitability of NR1^neo−/− cells treated with baclofen approached levels seen in wild type (WT) cells at baseline. (b) Synaptic events (evoked excitatory postsynaptic currents (eEPSCs)) were evoked by local stimulation within 100 μm of the cell body. Although group differences were not observed, baclofen reduced eEPSC amplitudes. The bottom panel shows average traces of eEPSCs evoked with 100-μs pulses; stimulus artifacts are omitted. (c) Baclofen reduced spontaneous synaptic events (sEPSC), in terms of frequency (left) and amplitude (right). The bottom panel shows segments of raw sEPSC recordings and averaged sEPSC amplitudes. Figures show mean±s.e.m. (^*P<0.05,^**P<0.01,^***P<0.001).