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. Author manuscript; available in PMC: 2012 Aug 2.
Published in final edited form as: Breast Cancer Res Treat. 2010 Sep 22;129(1):37–47. doi: 10.1007/s10549-010-1177-4

Fig. 4.

Fig. 4

CBD inhibits the expression of Id-1 and corresponding breast cancer proliferation and invasion in mouse 4T1 cells. (A) 4T1 cells were treated for 3 days with 1.5 μM CBD, proteins were extracted and analyzed for Id-1 expression. (B) 4T1 cells were collected and cell cycle analyzed using a desktop FACS Calibur with Cell Quest Pro software (BD Bioscience, CA). The distribution of cells in different cell cycle stages was determined according to their DNA content. (C) Invasion assays were carried out using the Boyden chamber assay. (*) indicates statistically significant differences from control (P < 0.05)