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Evaluation of genetic stability of the WN/DEN4Δ30 Lot 2 virus by the MARSH assay. The four panels show the ratios of hybridization signals of homogeneous reference DNA to cDNA from unpassaged virus (A), virus propagated 7 times in Vero cells (B), 6 times in SH-SY5Y human neuroblastoma cells (C) and 4 times in HeLa cells (D). The peaks on the ratio plots indicate the presence of mutations.
