Table 1.
Primers used to amplify specific regions from WN/dengue 4 chimera genomes, needed for preparation of micoarray fluorescent probes
| Primer names | Primer sequences (5′ to 3′) | Tm (°C) | PCR amplicon positions | Sub-array numbers |
|---|---|---|---|---|
| WN1F | AGTTGTTAGTCTGTGTGGACCGACAAG | 59 | 1-981 | 1 |
| WN958RT7 | ACGCGTTAATACGACTCACTATAGGCTCTGTTGCTCATTCCAAGGCAGT | 73 | ||
| WN947F | CTATTGCTTTTGGTGGCCCCAGCT | 60 | 924-2483 | 2 |
| WN2460RT7 | ACGCGTTAATACGACTCACTATAGGACTCCATGACGCCACACAACCCAT | 72 | ||
| WN2421F | GGAATCACTCTGTTTCTGGGCTTC | 56 | 2421-3543 | 3 |
| WN3543RT7 | ACGCGTTAATACGACTCACTATAGGCCATAGAAAAAGTTTCTGATGTGCCCG | 72 | ||
| WN3477F | AAAGAAGAGAACATGGTCAAATCACAGG | 58 | 3477-4581 | 4 |
| WN4642RT7 | ACGCGTTAATACGACTCACTATAGGAGGGGACGTCCCACAGGGCT | 72 | ||
| WN4519F | TGACCTTATGGTACATGTGGCAAGTG | 58 | 4519--6998 | 5 |
| WN6998rT7 | ACGCGTTAATACGACTCACTATAGGAGTCAGAATTGTGGTGGCTACTGCATAGAG | 73 | ||
| WN6836F | CACCATCATTGGTCTAATAGC | 49 | 6836-8635 | 6 |
| WN8606RT7 | ACGCGTTAATACGACTCACTATAGGGTTGTATCTGTCATGGCTAACTGAGTC | 71 | ||
| WN8060F | AATAGAGGAAGGAAGAACATTAAG | 51 | 8060-9617 | 7 |
| WN9617RT7 | ACGCGTTAATACGACTCACTATAGGCCTCTCATCTAGGGGCTTCACCA | 73 | ||
| WN9211F | ACACAAGAATCACTGAGGATG | 50 | 9211-10651 | 8 |
| WN10651RT7 | ACGCGTTAATACGACTCACTATAGGAGAACCTGTTGGATCAACAACACCAATCC | 71 |