Figure 2.  .

CN03-induces CTGF expression in HTM cells. Treatment of HTM cells treated with the Rho GTPase activator CN03, which is a cell permeable bacterial toxin (5 μg/mL for 24 hours under serum free conditions) leads to significant (*P < 0.05; **P < 0.01) increases in CTGF protein levels in cell lysates (A, B; n = 5) and conditioned medium (C, D; n = 3). Results are based on immunoblotting analysis with subsequent densitometric scanning. The lower panel in (C) indicates equal protein loading of conditioned media samples based on Coomassie blue staining profiles of SDS-PAGE gels.