Fluorescence images of competitive cellular uptake of RITC-labeled magnetic nanoparticles for 12 hours. A549 cells (HER2-negative, cytoplasmic membrane-labeled with PKH67 fluorescent linker, green) cocultured with HER2-overexpressing SKOV-3 cells were incubated with RITC-labeled magnetic nanoparticles (red). (A) LTVSPWY-PEG-CS-modified magnetic nanoparticles, (B) PEG-CS-modified magnetic nanoparticles, and (C) chitosan-modified magnetic nanoparticles.
Note: The cells were all stained with Hoechst 33342.
Abbreviations: RITC, rhodamine B isothiocyanate; PEG, poly(ethylene glycol); CS, chitosan.