Protein expression of (A) IR-β, and (B) IRS-1 in rat skeletal muscle. Equal amounts (40 mg/well) of protein were separated by SDS-PAGE and immunoblotted with IR-β or IRS-1 antibodies.
Notes: The animal groups are: chow-fed, untreated dietary obese, diet-to-chow, and fenofibrate-treated. Data are presented as the mean ± standard error. Data are normalized to that of control group and expressed as a percentage of the chow-fed (control) group. There were no significant differences in protein expression between any of the groups.
Abbreviations: C, chow-fed; V, untreated dietary obese; DC, diet-to-chow; F, fenofibrate-treated; IR-β, insulin receptor beta; IRS-1, insulin receptor substrate 1; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.