Table 1.
Sequences of primers and probes used in quantitative real-time qPCR
| Gene symbol | Amplicon size (bp) | Forward primer (5′…3′) | Probe (5′…3′) | Reverse primer (5′…3′) |
|---|---|---|---|---|
| Gapdh | 140 | gcatggccttccgtgttc | tacccccaatgtgtccgtcgtgga | gtagcccaagatgcccttca |
| Lys | 53 | tgcttcccgttacggttttga | cattccggaaatttg* | aacggcccggttcgat |
| Dap | 69 | cactggtggaagaagcgatct | tccgccggaatccata* | gcgccggaaggctttt |
| Phe | 79 | ggcccaaaacgaccttaatgg | ccggactgatcatcc* | gaaaatgcagacagcactctatgc |
| Thr | 57 | ccgatgccgtaaaagcaagtg | ctgtcagcaatatcc* | tggacatgatcgcagcaatga |
| Cacna1a | 140 | atctttcgttttcccggtgaa | ctgcacgccccacacacttctcag | cttgtagttgatctctgcaggcagta |
| Cacna1b | 140 | cttgcttcctgactttgggttt | tcttttcaagatgttgtctgtttccccctg | ccactgcttgacaggttcca |
| Cacna1c | 144 | gctatttgaaagcaagaacaaacca | cccacgagatatgcaaaagcaatgcaa | atgctgacacaactcacaaccat |
| Cacna1d | 142 | gcagagtgctgttctcctaaagg | tggactctgtcgtgctgggtgtctgt | cgatcacccattccagaacac |
| Cacna1e | 140 | gctgccacccacattatcaa | tgagtcaagcccatgagacgccc | tctgggtgggccagcat |
| Cacna1g | 140 | gcacctcgcatgccttctt | ctgaagagaacacagccctggagccc | cgagagccttggccacact |
| Cacna1 h | 140 | gtctcgttcctgtccatcatcac | cggcatccttccctctcacagtaccc | tgcatgggccagtactcctt |
| Cacna1i | 140 | cagagccagggctgaactg | cagaggccgccttgtccagca | aactaggagggaagttcgttcaca |
| Hcn1 | 140 | gtgccacaacgtgtcacctt | tccgacagatgtcctcgggagcc | tctgcatctgggtctgtatttagg |
| Hcn2 | 63 | gctgtgccccgcatgt | cggtggcctccgaggaagaatatgg | gggccgagtattgcacttga |
| Hcn4 | 140 | ctgagagtcttcggacctgtgtt | agccagctggacgccacagaacc | gacaagagacccctcactccttatc |
| Kcna1 | 140 | ttgccaattgcttcctcctt | taaggacacgtctcatctcatcacccctg | tgaaaactatgcgcatccttagg |
| Kcna2 | 79 | cccatgccaggcacaaag | tctgtgcttctcttcttctcctttgtggga | caccccatgtcatgaaattcag |
| Kcna3 | 140 | cgggagaaacaacagaaattcct | tgtcagacaccccttccagcgga | cacgggttgctatgggaact |
| Kcna4 | 156 | ttctggaggaaatgtaacaagttttg | tctatgccgaagcccgagagagacttc | cccattcttccaatgattgtca |
| Kcna5 | 142 | cccatcaaagtccggatcct | ctttccccgtttacccttcccaccc | aacggctcctgcgtcatg |
| Kcna6 | 140 | cacgaggccactctggaaaa | acttctctggaggatgagacccccgc | ccgtctcagtcactgcttgtg |
| Kcnb1 | 140 | ggaaggccccccttacaata | agcaccccaatcccatcagctcaga | tccttgaaagatggagcaatcc |
| Kcnc1 | 147 | ccaatgtgaccaagtttcataacaa | cctgcagtccagggccaccaga | cagttctcgccttgatgaaggt |
| Kcnc2 | 140 | atagcctaactataacccccctgtatc | cttttgtgaccatctcaaggtgcatgcc | ttcaagtctatccccagatcagttg |
| Kcnc3 | 248 | gcagcctgagcagcattgt | caccccccctggctctcccc | gcatcgtctcttttgcatattattg |
| Kcnc4 | 140 | cttcctgcccatccttggta | acgcctaggctgggcccactgt | tttattggtactcgagcacagcat |
| Kcnd1 | 140 | ctgacccctgggagagatagaa | atcgcctccttctctgctgtaactcctttg | ggagacagggaacctgagatga |
| Kcnd2 | 152 | agctgagtgacagggaaaagaact | ctgaccctcgggaagacctggctt | tgctctagaagcataaagtgacaaca |
| Kcnd3 | 140 | tgggagggatggtatatttttga | caatgttttccttagaggtcatgcgctcc | ggtggtaagcaaagcccaataa |
| Kcnf1 | 140 | gggttgggtgtggagttttg | tgtccaggctacaccccattgctgt | ggatttaacccaatcaccatgac |
| Kcng4 | 140 | cgcccctactttcctgactgt | tgccccagcatctactctaaagtgcatctt | aggagcatgtgtgtgcatctg |
| Kcnh1 | 140 | cacccccaccccaatga | tggagactgtccaccatccaagttgc | atgtttgtatgagaagcctcaggat |
| Kcnh2 | 144 | tgcccactgtggacatgtg | cagggcattacctgaccgctctcct | cagggtattgcaggaggagatg |
| Kcnma1 | 140 | cagaactgcaacatttcaaacataaa | cccaggtggtacaatcacatcctgcc | aagtctcttttcggcatctgtgt |
| Kcnn1 | 141 | gggatcctgatgtcaaagtaagga | tacagacgagcagagtcaccacggtca | cccatgcctgtgaggtgtct |
| Kcnn2 | 140 | aaaggatcgtcaccctggaa | tagcattcatgccctccctgggc | gacatgcttgtcgtagttctccat |
| Scn1a | 149 | gcttcagaatttcttttcctcaaaa | cagaagcagaccgtaggcacctctttttct | gcagttcatgaatacggtttgc |
| Scn1b | 119 | tgcatgatgggtgaagcaat | ccccaccctgcttttgctgcct | gcctatgtgcaaatcagcaaaa |
| Scn2a1 | 140 | ttgaatgcatgcgggaaaat | ctaccggtaagccacattacaatcaagcca | catctcgaggatagaaacacagaca |
| Scn2b | 140 | ataggaatggcttggtgcagtt | cagccttgctgtgcctcctgctg | tatgtacaatcgcatcagcaagct |
| Scn3a | 175 | cacgggctctgtcctttctaa | caggaaccaagtgggaaagttgtgcaa | gccattccctgcatgca |
| Scn3b | 140 | tataaatgagaaagccctggttctg | aattcactcagtcaatgca* | gcctaacctagtgctcgacaca |
| Scn4b | 140 | ggatgcccaaatgagggatt | tattttgtcccaggttggagtccctgctc | ccagaggactaaaccatgcataaga |
| Scn8a | 140 | cagcaggtcgagaaatgtctttc | tatctccttccttacccctccactcgttcc | ctcaagcatttctgccatttcc |
| Adcyap1 | 141 | tggtgaaactgggctctagtga | agtctaacttagtgttcacgtgg* | cagtttagtaaggctcttggcattt |
| Cck | 140 | tccttctggccgcatgtcc | cttaagaacctgcagagcctggacccc | tggcccactacgatgggtat |
| Coch | 71 | gcccttagaaactcaggaaagaaga | ttgtcatggattaacattg* | tccacttgaatgcatatttgaactc |
| Col5a1 | 140 | gaccccatgcccagagaga | acggagccgaatcacatgacctagcc | cggccaacggtccattaac |
| Crh | 140 | cgcccatctctctggatctc | agttagctcagcaagctcacagcaacagg | ttggccaagcgcaacatt |
| Crhbp | 151 | ggccggcttcagtgattg | ccaggccttgaacacgtgcacaca | caaaaggaagcaaaattggaactc |
| Grin2c | 140 | gttcctgctggactggattctg | cctctgccttcttctttgaaattctggcc | ggtcataagctggccatgga |
| Gsg1l | 140 | gggtcagattccagaccaatga | catgctcaggccccatcccagt | agacaaagttgacctggcagagt |
| Hspb8 | 142 | gggcaggtcttatatccccatat | tccttcaccacacaacacagtgcctttt | ggaccttagccagagaattaactga |
| Nrn1 | 140 | gccggtttcacctgctattg | cgatgcccctcgttcaggtcca | taacattgattacccccttgtgatc |
| Sema3a | 140 | agtttctatagatgctccattccagtt | tcaccatcatcctcaagtgcaagatttcc | gacctggcaaaaagcatgct |
| Spp1 | 107 | cagtcggatgtgatcgatagtca | tccaaagccagcctggaacatcaga | ttactcttagggtctaggactagcttgtc |
| Sst | 140 | ttctggaagacattcacatcctgtt | ttgttgtcctagccagacctctgatccctc | tcttcaatttctaatgcagggtcaa |
| Tacr3 | 141 | gtgtgcagataaacactatcaaaaatgaat | tcacaacaggattgccccaaaagcc | aaacatgcacacaatgcttcgt |
| Gad1 | 158 | tcatgttatggaaatcttgcttcag | agccttcgctcttctctttcccccc | cgagtcacagagattggtcatatactact |
| Gad2 | 140 | caactaagtcccaccctaagaaatct | tgtgatggcacacaaaatgcttcatctct | tccaagaccctgtagagtcaatacc |
| Slc17a6 | 141 | ttgcttgtggtagcagtgatttc | tgcgctgataagaatgcacatcccaat | aaatacccaaaactgacaggtaaaca |
| Slc17a7 | 140 | ccgggccttgaccttagc | ccaaaatggctctgctcaccccg | cctcgagccgctgaattaat |
| Slc6a5 | 140 | ctgtaaactgcctgcagaggaa | gcaggtgaccagctgccttacaggtg | cagcagcccaatgatcaaaa |
*Conjugated with MGBNQF (Applied Biosystems) instead of Iowa Black FQ (Integrated DNA Technologies) at 3′.