Table 3.
Potential miRNA targets found only in cotyledons at different seed developmental stages
| miRNA | Tissue | Target | Target Annotation | C.Site | Location | Category | TP100M | P-Value |
|---|---|---|---|---|---|---|---|---|
| gma-miR171 |
C25 |
Glyma15g01820.1 |
Protein tyrosine kinase |
1359 |
CDS |
1 |
24.2379 |
0.0013 |
| |
C300 |
Glyma12g08490.1 |
Putative methyltransferase |
434 |
CDS |
1 |
12.3961 |
0.0462 |
| gma-miR394 |
C100 |
Glyma06g13230.3 |
Ferredoxin related protein |
1070 |
CDS |
1 |
104.088 |
0.027 |
| |
C100 |
Glyma06g13230.2 |
Ferredoxin related protein |
685 |
CDS |
1 |
104.088 |
0.027 |
| |
C100 |
Glyma14g25810.1 |
GTPase-activating protein |
1013 |
CDS |
1 |
57.8265 |
0.0035 |
| |
C100 |
Glyma06g13230.1 |
Ferredoxin related protein |
952 |
CDS |
1 |
104.088 |
0.027 |
| gma-miR398 |
C100 |
Glyma03g40280.2 |
Copper/Zinc superoxide dismutase |
156 |
5' UTR |
2 |
57.8265 |
0.0379 |
| |
C100 |
Glyma03g40280.3 |
Copper/Zinc superoxide dismutase |
156 |
5' UTR |
2 |
57.8265 |
0.0379 |
| gma-miR1509 |
C300 |
Glyma18g03980.2 |
No Functional Annotation |
2155 |
3' UTR |
0 |
7.43768 |
0.0041 |
| gma-miR1513 |
C300 |
Glyma08g27950.1 |
F-box domain containing protein |
190 |
CDS |
0 |
29.7507 |
0.0402 |
| gma-miR1514 |
C25 |
Glyma07g05360.2 |
No apical meristem protein |
773 |
CDS |
0 |
16.1586 |
0.0371 |
| gma-miR1515 |
C300 |
Glyma09g02920.1 |
PAZ domain containing protein |
2750 |
CDS |
0 |
57.0223 |
0.003 |
| gma-miR1531 |
C100 |
Glyma16g33400.1 |
Serine protease inhibitor family |
536 |
3' UTR |
3 |
46.2612 |
0.0414 |
| gma-miR1532 |
C25 |
Glyma10g28900.1 |
Universal stress protein family |
626 |
3' UTR |
4 |
4.03965 |
0.015 |
| gma-miR1535 |
C25 |
Glyma08g22920.1 |
Ribose 5-phosphate isomerase |
168 |
CDS |
1 |
60.5947 |
0.0242 |
| gma-miR2109 |
C25 |
Glyma03g14900.1 |
LRR containing protein |
46 |
CDS |
0 |
88.8723 |
0.0165 |
| gma-miR4357 |
C300 |
Glyma13g01500.1 |
Alg9-like mannosyltransferase |
779 |
CDS |
1 |
22.3131 |
0.0089 |
| |
C300 |
Glyma01g35530.1 |
Transferase family protein |
522 |
CDS |
1 |
9.91691 |
0.0114 |
| gma-miR4369 |
C25 |
Glyma19g43800.1 |
Calcineurin-like phosphoesterase |
1257 |
3' UTR |
4 |
4.03965 |
0.0468 |
| gma-miR4371 |
C300 |
Glyma15g08400.4 |
No Functional Annotation |
452 |
3' UTR |
0 |
17.3546 |
0.0017 |
| gma-miR4380 |
C300 |
Glyma06g10840.1 |
MYB family transcription factor |
630 |
CDS |
1 |
9.91691 |
0.0347 |
| gma-miR4387 |
C300 |
Glyma08g43670.1 |
Uncharacterized conserved protein |
1501 |
CDS |
0 |
4.95846 |
0.0068 |
| gma-miR4390 |
C100 |
Glyma15g06380.1 |
Dynamin family protein |
287 |
CDS |
3 |
104.088 |
0.0251 |
| gma-miR4398 |
C25 |
Glyma02g25150.1 |
Integrase domain containing protein |
199 |
5' UTR |
4 |
4.03965 |
0.0444 |
| gma-miR4402 |
C300 |
Glyma02g18090.1 |
Lectin domain containing protein |
783 |
CDS |
4 |
2.47923 |
0.0293 |
| gma-miR4403 |
C300 |
Glyma19g36500.1 |
No Functional Annotation |
1860 |
3' UTR |
4 |
2.47923 |
0.0484 |
| gma-miR4408 |
C25 |
Glyma16g34800.1 |
No functional annotation |
434 |
CDS |
1 |
68.674 |
0.0057 |
| gma-miR4409 |
C25 |
Glyma16g04060.3 |
BTB/POZ domain containing protein |
1304 |
3' UTR |
0 |
12.1189 |
0.0144 |
| gma-miR4415 |
C100 |
Glyma02g20490.2 |
Transferase family protein |
502 |
CDS |
1 |
57.8265 |
0.0463 |
| |
C100 |
Glyma02g20490.1 |
Transferase family protein |
502 |
CDS |
1 |
57.8265 |
0.0463 |
| |
C100 |
Glyma15g12430.1 |
Transferase family protein |
464 |
CDS |
1 |
57.8265 |
0.0463 |
| gma-miR4416 | C100 | Glyma01g44970.1 | BTB/POZ domain containing protein | 434 | CDS | 0 | 161.914 | 0.0318 |
CDS: Coding Sequence; UTR: Untranslated Region; TP100M: Transcripts per 100 million; C.site (Cleavage site): Nucleotide number from 5’ end of cDNA; P-value ≤0.05 using Cleaveland pipeline.