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Time course analysis of vIL-10 accumulation in wild-type tobacco leaves following infiltration with bacteria carrying different expression constructs. ELISAs were used to determine vIL-10 levels in leaf protein extracts at 3, 4, 5 and 6 days post infiltration (dpi), expressed as μg/g FW. Vector abbreviations correspond to those in Figure 2. The insert show the phenotype of the infiltrated leaf tissue, which was mostly necrotic at 6 dpi (pTRA_e35STX).
