Figure 5.
Comparison of DsRed and vIL-10 accumulation levels. (A) Cell suspension cultures from different lines were induced with 10 μM Ahtc and 2-ml aliquots were analyzed daily from 3 to 7 dpi. The accumulation of vIL-10 in cell pellet extracts was determined by ELISA. (B) DsRed accumulation was determined in callus and cell suspension culture extracts from each transgenic line arrayed in 96-well plates using a fluorometer. The data reported are means ± SD from three independent experiments. (C) Linear regression between the yields of IL-10 (in suspension cultures at 7 dpi) and DsRed (in callus) for nine clones. The correlation coefficient R2 is indicated inside the plot.