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. 2012 Jun 8;12:100. doi: 10.1186/1471-2180-12-100

Figure 2.

Figure 2

Activation of NF-κB and up-regulation of IL-8 expression in HT-29 cells byC. butyricumwere enhanced in the presence of IL-10 antibody.(A) Immunoblot showing levels of NF-κB (p50/p105 subunits) and IκB in cells compared with the control. (B) IL-8 secretion in response to C. butyricum in control and anti-IL-10 treated cells. (C) IL-8 transcript levels as measured using real-time PCR. Results are mean ± SE for three experiments. *, P < 0.01 compared to the control without IL-10 antibody treatment (C- vs. C + and T- vs. T+). C: levels of NF-κB, IκB or IL-8 in control HT-29 cells. T: levels of NF-κB, IκB or IL-8 in HT-29 cells treated with C. butyricum.