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. 2012 Aug 2;8(8):e1002822. doi: 10.1371/journal.pgen.1002822

Figure 2. H2Bub1 depends on Cdk9 activity and Spt5 phosphorylation.

Figure 2

(A) Immunoblots of whole-cell extracts from wild-type (wt) (JS78) or AS mutant strains (LV7, LV77, LV42), as indicated, grown in the absence (−) or presence (+) of 20 µM 3-MB-PP1, added 20 min prior to harvest. Antibodies are indicated at right. (B) Immunoblots of extracts from indicated strains probed for total histone H2B and H2Bub1, as indicated at right. “T212A” and “T212E” denote strains cdk9-T212A (HD7-24) and cdk9-T212E (HG127). (C) Immunoblots of extracts from wild-type (JS78) or indicated spt5 mutant strains.