Figure 6. Cdk9 activity towards multiple substrates is required for abnormal morphologies of H2Bub1-deficient cells.
(A) Quantification of abnormal septation patterns in the indicated htb1-FLAG strains (JTB378, JTB351, JTB379, JTB353, JTB380, JTB355). Error bars represent standard deviations from 2 independent experiments; at least 200 cells were counted in each. (B) As in (A) for the indicated cdk9as strains (KL289, KL291, KL293). Cells were grown in the presence of either DMSO or 3-MB-PP1 as described in Figure 5B. (C) As in (A) for the indicated htb1-FLAG strains (MS260, MS256, MS261, MS257, MS272, MS259). (D) Flocculation of indicated strains (JS78, JTB67-1, JTB351, JTB353, KL291, KL293, MS259) was quantified as described in Materials and Methods. (E) Growth rates in liquid rich medium (YES) were measured for strains analyzed in (D).