Figure 5. mir-71 acts cell-autonomously to promote AWC^ON.

(A, B) Projections of wild-type animals expressing an integrated str-2p::GFP transgene (green) and an unstable transgenic array containing odr-3p::mir-71 and odr-1p::DsRed (red). AWC neurons with co-expression of GFP and DsRed appear yellow. Arrows, AWC cell body; arrowheads, AWB cell body; scale bar, 10 µm. (C, E) AWC phenotypes of wild type (C), nsy-4(ky627), and nsy-5(ky634) mutants (E) expressing the transgene odr-3p::mir-71; odr-1p::DsRed in both AWC neurons. + and − indicate the presence and absence of the transgene odr-3p::mir-71, respectively. (D, F) AWC phenotypes of wild-type (D) and mutant (F) mosaic animals expressing the transgene odr-3p::mir-71; odr-1p::DsRed in one AWC neuron. Two independent transgenic lines were analyzed in wild type, nsy-4(ky627), and nsy-5(ky634) mutants in (C–F). Results from two independent lines were similar and thus were combined in (E, F). Z-test was used to calculate p values. (G) Color codes for AWC neurons in (A), (B), (D), and (F).