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Lysates of uninfected (0 hpi) or infected HeLa cells were prepared in RIPA buffer (left panel) or by direct lysis in 8M urea (right panel) at the indicated times, separated by SDS-PAGE and probed with antibodies to the p65/RelA subunit of NFκB, cyclin B1, nectin-1, or RFX5 as indicated. Equal loading for each blot was monitored by blotting for Erk 1/2, but only the loading control for nectin-1 is shown.
