Figure 5. CPAF is autocatalytically cleaved into its active form in Chlamydia-infected cells.

Lysates of uninfected (0 hpi) or infected HeLa cells were prepared in RIPA buffer (left panel) or by direct lysis in 8M urea (right panel) at the indicated times, separated by SDS-PAGE and probed with antibodies to the C-terminal fragment of CPAF (CPAFc) and α-tubulin (loading control).