Table 1. qRT-PCR confirmation of genes of interest identified in the dataset.
24 h | 48 h | 72 h | Pearson correlation (excluding Mock) | ||||||||
Mock | Bystander | Infected | Mock | Bystander | Infected | Mock | Bystander | Infected | |||
ZBED2 | Microarray | 1.00 | 0.86 | 3.28 | 1.00 | 0.74 | 3.03 | 1.00 | 0.73 | 2.86 | 0.87 |
PCR | 1.00 | 0.78 | 1.68 | 1.00 | 0.74 | 2.16 | 1.00 | 1.13 | 3.53 | ||
MYOF | Microarray | 1.00 | 0.87 | 3.77 | 1.00 | 0.71 | 2.74 | 1.00 | 0.66 | 2.06 | 0.94 |
PCR | 1.00 | 1.20 | 2.14 | 1.00 | 0.82 | 2.60 | 1.00 | 0.63 | 1.69 | ||
GJB2 | Microarray | 1.00 | 1.03 | 4.78 | 1.00 | 0.65 | 3.59 | 1.00 | 0.92 | 3.38 | 0.83 |
PCR | 1.00 | 0.79 | 2.45 | 1.00 | 0.92 | 6.36 | 1.00 | 1.23 | 2.41 | ||
GJB6 | Microarray | 1.00 | 1.03 | 3.29 | 1.00 | 0.82 | 3.21 | 1.00 | 0.96 | 2.62 | 0.79 |
PCR | 1.00 | 1.32 | 1.59 | 1.00 | 1.24 | 3.80 | 1.00 | 0.91 | 2.32 | ||
TRIM22 | Microarray | 1.00 | 1.20 | 0.50 | 1.00 | 1.30 | 0.88 | 1.00 | 1.77 | 1.50 | 0.87 |
PCR | 1.00 | 1.95 | 0.24 | 1.00 | 1.11 | 0.60 | 1.00 | 1.40 | 0.93 | ||
CLC | Microarray | 1.00 | 1.52 | 0.35 | 1.00 | 1.90 | 0.36 | 1.00 | 2.41 | 0.29 | 0.95 |
PCR | 1.00 | 3.01 | 0.08 | 1.00 | 1.09 | 0.14 | 1.00 | 2.41 | 0.14 | ||
DFNA5 | Microarray | 1.00 | 1.01 | 1.55 | 1.00 | 0.96 | 2.60 | 1.00 | 1.02 | 3.11 | 0.99 |
PCR | 1.00 | 0.90 | 2.06 | 1.00 | 1.14 | 8.11 | 1.00 | 0.85 | 8.80 |
Expression levels of ZBED2, MYOF, GJB2, GJB6, TRIM22, CLC and DFNA5 were confirmed by qRT-PCR. The Pearson correlation coefficients indicate that the level of similarity between qRT-PCR quantification and microarrays is very high for all studied candidates.