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. 2012 Aug 2;8(8):e1002847. doi: 10.1371/journal.ppat.1002847

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(A) Virus titration by TCID₅₀. Homogenized liver and spleen samples, as well as blood samples, from animals (n = 3) infected with 10 ffu of either recombinant (rZEBOV and rREBOV) or chimeric (rZEBOV-RGP and rREBOV-ZGP) Ebola viruses were analysed at day 5 post-infection for viral load by calculating the tissue culture infectious dose (TCID₅₀) using the Reed and Muench method [54]. The values for each animal as well as the mean for each virus group are shown. (B) Evaluation of virus infection in organs by immunohistochemistry. The presence of viral antigen was detected in liver and spleen samples from infected animals by immunohistochemical straining using a cross-reactive anti-ZEBOV VP40 antibody.