Figure 6. miR-7 inhibits breast cancer cell growth, local invasion in vivo.

(A) Primary tumor growth upon orthotopic injection of 1.0×10⁶ MDA-MB-435s cells infected as indicated. n = 12 per group per time point. (B) H&E staining of primary tumors and mice lungs after orthotopic injection. Arrows: tumor cells venous (a), muscle (b) invasion and lung metastasis foci (d). (scale bar: 100 µm) (C) FAK, VIMENTIN, FIBRONECTIN, E-CADHERIN stained primary tumors after orthotopic injection. Arrow indicate metastatic foci (scale bar: 50 µm). (D) Evaluation of nuclear BrdU incorporation and TUNEL positive (apoptotic) nuclei in MDA-MB-435s tumors. ***, p<0.001.