Skip to main content
. 2012 Aug 2;7(8):e42137. doi: 10.1371/journal.pone.0042137

Figure 3. RNA analysis of gut tumors in the transgenic mice.

Figure 3

A) Polyacrylamide Northern blot analysis of expression of miR-143 and miR-145 of gut tumors. Ten µg of total RNA of small intestines and colons of Tg/APC and their non-transgenic littermates (W/APC) was applied in each lane. The membrane was hybridized with the probes for miR-143(upper panel), miR-145 (middle panel) and 5S rRNA (lower panel). Individual identification number is presented. B) Quantitative Real-Time PCR (qRT-PCR) analysis of the mouse endogenous pri-miR-143 and pri-miR-145. The expression of mouse pri-miR-143 and pri-miR-145 of the small intestine tumors from Tg/APC (gray bars, n = 5) and their non-transgenic littermates (W/APC) (open bars, n = 4) was examined. Each set of primers cover the region that contains pre-miR-143 or pre-miR-145. The data are representative of three independent experiments and are presented as the mean ± SEM. C) qRT-PCR analysis of the mouse endogenous pri-miR-143 and pri-miR-145 of the colon tumors. The expression of mouse pri-miR-143 and pri-miR-145 of tumors from Tg/APC (gray bars, n = 3) and their non-transgenic littermates (W/APC) (open bars, n = 3) was examined in the same way as B).