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. 2012 Aug 2;7(8):e42194. doi: 10.1371/journal.pone.0042194

Figure 3. Neocortical arterioles of IP3R2− /− slices do not respond to electrical field stimulation.

Figure 3

A. Responses of arterioles in 95% O2 following electrical field stimulation. Cortical slices from IP3R2+/+ (open circle) and IP3R2− /− mice (closed circle) were treated with electrical field stimulation (EFS) of 100 Hz trains of 200 ms at 0.2 Hz for 4 minutes as indicated by the dark bar. Arteriole diameter was measured every 5 min during the experiment. IP3R2+/+, n = 15; IP3R2− /− n = 15. **, P<0.01, IP3R2+/+ compared to IP3R2− /−. B. Pretreatment with U-46619 for 30 min was followed by electrical stimulation and arteriole diameters of IP3R2− /− were compared to IP3R2+/+. IP3R2+/+, n = 15; IP3R2− /− n = 14. **, P<0.01; ***, P<0.001. C. Blockade of Group I mGluR with MPEP and JNJ prevents arteriole responses to electrical stimulation in IP3R2+/+ slices (inverted triangles) while a 20% O2 environment does not alter responses of naïve IP3R2+/+ or IP3R2− /− arterioles. IP3R2+/+, n = 14; IP3R2− /− n = 12, IP3R2+/+ with MPEP/JNJ, n = 9. **, P<0.01; ***, P<0.001.