FIGURE 2.

1411HP cells are resistant to cisplatin. A, TGCT cell lines were treated with 12 μm cisplatin for 24 h and then cell death was determined by cytometry using TUNEL assay. Percentages of apoptosis are indicated. B, cisplatin-induced apoptosis was also determined by Western blot analysis of PARP cleavage at 24 h or at the indicated time points. C, NTERA2 and 1411HP cells were treated with cisplatin and the protein expression of Noxa was analyzed by Western blot. The levels of α-tubulin were also analyzed to assure equal loading. D, NTERA2 and 1411HP were transfected with a luciferase reporter plasmid containing the Noxa promoter. Following treatment with cisplatin, cell extracts were analyzed for the relative luciferase activity. Histograms represent the mean ± S.D. of three independent experiments.