TABLE 1.

Summary of thermodynamic and kinetic parameters of the RhoGDI interaction with GDP- and GMPPNP-associated RhoA

Protein	kon	koff	Kd
	μm−1 s−1	s−1	m
RhoA·GDP	2.42 ± 0.15	0.54 ± 0.01	0.17 × 10−6
RhoA·GMPPNP	3.8 ± 1.9	8.77 ± 0.73	2.5 × 10−6
CFP-RhoA·GDP-GG	37a	(2.0 ± 0.02) × 10−4	(5.4 ± 2.5) × 10−12
CFP-RhoA·GMPPNP-GG	1.6a	(4.6 ± 0.03) × 10−3	(2.9 ± 0.65) × 10−9
RhoA·GDP-GNBD	10.7 ± 0.7	(5.8 ± 0.3) × 10−2	(2.0 ± 0.15) × 10−9
RhoA·GDP-F	NDb	ND	(2.5 ± 1.1) × 10−9

^a Calculated from k_on = k_off /K_d.

^b ND, not determined.