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. 2012 Jun 14;287(32):26606–26617. doi: 10.1074/jbc.M112.363259

FIGURE 6.

FIGURE 6.

A, an ensemble of NMR conformers of B2088 in LPS (peptide/LPS molar ratio = 1:20) (AI) and in DPC-POPG mixed micelles (peptide/DPC/POPG = 1:30:13) (AII). Backbones of primary and secondary chains are labeled in blue and cyan, respectively. The side chains of charged amino acids are shown in orange. The Lys-9 side chain linking the primary and secondary chains is highlighted in gold. B, 1H,15N HSQC spectra of B2088 in aqueous solution (BI), LPS (BII), and DPC-POPG mixed micelles (BIII). In BI and BII, the chemical shifts of spin residing in equivalent positions in both chains are degenerate, labeled in blue. In BIII, the secondary chain that is more tightly structured is labeled in cyan with a prime. Additional peaks coming from a minor conformation are highlighted in pink. C, 1H,15N HSQC spectra of B2088 peptides in POPG-POPE liposomes. CI, peptide/POPG ratio equals 1:1. CII, peptide/POPG ratio equals 1:12. CIII, peptide/POPG ratio equals 1:13. All peaks are labeled at the same position to show the relative movement of chemical shifts.