FIGURE 2.

Expression of TRPC isoforms and A₁AR. A, TRPC1, TRPC3–7 proteins were expressed in cultured atrial and ventricular myocytes as well as in whole heart as the positive control (n = 3 primary cultures). B, A₁AR mRNA was identified by RT-PCR in cultured myocytes and whole heart (n = 2 primary cultures). PCR product of the predicted size was 318 bp. β-Actin was amplified as a positive control. The negative control (Ctrl(−)) contained water instead of DNA.