Skip to main content
. 2012 Jun 18;287(32):26702–26714. doi: 10.1074/jbc.M111.316869

FIGURE 4.

FIGURE 4.

ISRE element of PML promoter gene is involved in basal transcription of PML gene. Shown is luciferase reporter gene activity under the control of PML gene promoter (−809 bp/+633 bp relative to transcription start) in HeLa cells with an intact or deleted (−595 bp/−628 bp) ISRE DNA binding site (cells were harvested 24 h after transfection; the average values representing three independent experiments are given as arbitrary units (AU); error bars represent S.E.) (A), with or without IL6-depleting antibody (IL6 AB; 2 μg/ml; average values representing two independent experiments are given as arbitrary units; error bars represent S.E.) (B), and with or without IL6 treatment (5000 pg/ml; average values representing two independent experiments are given as -fold induction; error bars represent S.E.) (C). Asterisks (*, **, and ***) represent p values <0.05, <0.01, and <0.005, respectively. D, shown is evaluation of STAT3 binding to PML ISRE element in untreated BJ, HeLa, and U2OS cells and in HeLa and U2OS cells treated with IL6 for 0.5, 1, and 6 h in HeLa and 48 h in U2OS cells, respectively, quantified with RT-qPCR after chromatin immunoprecipitation. The values quantified with RT-qPCR represent average of two independent experiments performed in triplicate and are expressed as -fold induction relative to HeLa untreated cells.