FIGURE 7.

E328V mutant phenotype can be partially rescued by cis-effect of N196K substitution without changes to Cox10 oligomerization. A, Western blot analysis of the mitochondria from the BY4743 cox10Δ cells expressing either WT Cox10-13×Myc or indicated mutant forms of the protein. 10 μg of mitochondrial proteins were separated by SDS-PAGE and detected with antibodies against Myc epitope or porin. B, respiratory growth of the BY4743 cox10Δ cells transformed with indicated constructs. Transformants were handled as in Fig. 2C. Pictures of the plates were taken after 2 (glucose) or 4 and 8 (glycerol lactate) days of incubation at 30 °C. C, CcO-specific activities of mitochondria isolated from indicated transformants were determined as described in Fig. 6D. D, immunoblot analysis of the steady-state levels of Cox1, Cox2, Cox3, Sdh2, and porin analyzed in 10 μg of transformant-derived mitochondria. E, oligomeric state of Cox10-13×Myc and its mutant forms was analyzed by BN-PAGE as described above.