FIGURE 8.

Synergistic effect of increased Cox1 synthesis and Cox10 expression restores respiration in coa2Δ cells. A, respiratory growth of the WT cells with either full-length or truncated Cox1 or respective coa2Δ cells bearing YCp-Cox10 (low copy), YEp-MSS51 (high copy), or co-expressing both constructs. Cells were pre-cultured and tested as described in Fig. 2C except that the plates were incubated at either 30 or 37 °C. B, BN-PAGE analysis of mitochondria derived from coa2Δ transformants described in A. The exposure shown does not reveal the low abundance of the Cox10 oligomer with overexpression of MSS51. C, growth of coa2Δ cells co-expressing YEp-MSS51 and YCp-COX10 or its mutant alleles was tested as in A.