FIGURE 1.

Nrdp1 up-regulates Arg1 expression in macrophages. A, two-dimensional DIGE analysis of total cell lysates of peritoneal macrophages from Nrdp1 transgenic (Nrdp1-TG) and wild-type (WT) mice. Fifty μg of fraction protein was labeled with Cy3 (WT macrophages) or Cy5 (Nrdp1-TG macrophages), respectively. Differentially expressed Arg1 protein is shown. B, Arg1 mRNA expression in peritoneal macrophages from Nrdp1-TG and WT mice was detected by Q-PCR. C, Arg1 protein expression in peritoneal macrophages from Nrdp1-TG and WT mice was detected by Western blot. Results are representative of three experiments. *, p < 0.05 as compared with WT macrophages.