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. 2012 Jun 15;287(32):26740–26748. doi: 10.1074/jbc.M112.383265

FIGURE 6.

FIGURE 6.

Nrdp1 enhances IL-4-induced ubiquitination of C/EBPβ. A, peritoneal macrophages from Nrdp1-TG mice and WT mice were stimulated with 10 ng/ml IL-4 for 1 h. Cell lysates were immunoprecipitated (IP) with anti-C/EBPβ antibody. Ubiquitination of C/EBPβ was examined by immunoblot (IB) using anti-ubiquitin (UB) antibody. B, immunoblot analysis of C/EBPβ in peritoneal macrophages from Nrdp1-TG mice were left untreated or treated with IL-4 (10 ng/ml) for 24 h. C, NIH-3T3 cells were transfected with pcDNA3.1-C/EBPβ, pcDNA3.1-Nrdp1, along with pcDNA3.1-HA-ubiquitin (HA-UB) or pcDNA3.1-HA-ΔK63-ubiquitin (HA-ΔK63UB) and left untreated or treated for 15 min with IL-4 (10 ng/ml). Cell lysates were then immunoprecipitated with anti-C/EBPβ antibody. Ubiquitination of C/EBPβ was examined by anti-HA antibody. Results are representative of three independent experiments.